Composite

Part:BBa_K3805924:Design

Designed by: Han Yu   Group: iGEM21_BNU-China   (2021-09-30)


T7-RBS1- AgrC-T7 terminator-RBS2- AgrA-T7Te terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 814
    Illegal SpeI site found at 1355
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 814
    Illegal SpeI site found at 1355
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 814
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 814
    Illegal SpeI site found at 1355
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 814
    Illegal SpeI site found at 1355
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We combine those parts together to make a complete access to manipulate the activation of P2.



Source

T7:https://parts.igem.org/Part:BBa_I719005 RBS1:https://parts.igem.org/Part:BBa_J61115 AgrC:The lab of Beijing Normal University

T7terminator::https://parts.igem.org/Part:BBa_B0013 RBS2: :https://parts.igem.org/Part:BBa_J61102 AgrA:The lab of Beijing Normal University

T7Te Terminator:https://parts.igem.org/Part:BBa_B0012


References